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KMID : 0370219960400030335
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Yakhak Hoeji
1996 Volume.40 No. 3 p.335 ~ p.339
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Induction of beta-Glucosidase and alpha-Rhamnosidase of Bacteroides JY-6 by Flavonoid Glycosides
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장일성/Jang IS
박종배/김동현/Park JB/Kim DH
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Abstract
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Optimal medium for growth and glycosidases production of Bacteroides JY-6, an human intestinal bacterium, was general anaerobic medium or tryptic soy broth containing sodium thioglycolate and ascorbic acid. By cocultivation of Staphylococcus R-48, Bacteroides JY-6 could be cultured in LB broth unable to culture JY-6. Heated Staphylococcus R-48 was also the inducer of the production of Bacteroides JY-6 glycosidases. These glycosidases were induced well by natural flavonoid glycosides, such as poncirin, naringin and rutin, but were not by synthetic substrates, p-nitrophenyl beta-D-glucopyranoside and p-nitrophenyl alpha-L-rhanmopyranoside.
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